Navigation Links
Restriction fragment length polymorphism


In molecular biology, the term restriction fragment length polymorphism (or RFLP) is used in two related contexts: as a characteristic of DNA molecules (arising from their differing nucleotide sequences) by which they may be distinguished, and as the laboratory technique which uses this characteristic to compare DNA molecules. The technique is utilized in genetic fingerprinting and paternity testing.

Method

Usually, DNA from an individual specimen is first extracted and purified. Purified DNA may be amplified by Polymerase Chain Reaction (PCR). The DNA is then chopped into restriction fragments by endonucleases, which only cut where there are specific DNA sequences recognized by the enzymes. The restriction fragments are then separated according to length by agarose gel electrophoresis. The resulting gel may be enhanced by Southern blotting.

Result

The distance between the locations cut by restriction enzymes (the restriction sites) varies between individuals: so the length of the fragments varies, and the position of certain gel bands differs between individuals (thus polymorphism). This can be used to genetically tell individuals apart. It can also show the genetic relationship between individuals, because children inherit genetic elements from their parents. It is also used to determine the relationships among species.

See also


'"/>


(Date:1/14/2019)... ... January 14, 2019 , ... Join ... (4pm GMT) to hear about key topics in data quality, including:, ... ,     Patient engagement ,     Smartphone and wearable based performance measures , ...
(Date:1/8/2019)... ... January 07, 2019 , ... Join industry experts Julie Peacock, Client Services ... webinar on Thursday, January 17, 2019 at 1pm EST. , This webinar ... the clinical trial process. Featured speakers will also present a case study of a ...
(Date:1/7/2019)... ... January 07, 2019 , ... Franz ... Graph Database technology for Knowledge Graphs solutions, announced that it was recognized by ... . DBTA’s data and information management recognition list includes newer approaches leveraging ...
Breaking Biology News(10 mins):
Other biology definition