Navigation Links
His-tag


Contents

Structure

A his-tag consists of approximately six histidine residues near the N- or C-Terminus of a Protein. The total number of histidine residues may vary. It may also be followed by a suitable amino acid sequence that facilitates a removal of the his-tag.

Purpose

Purification

The his-tag can be used in affinity chromatography together with a column that has nickel bound to it. Histidine is a good ligand for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole.

Detection

The his tag can also be used in detection of the protein via anti-his-tag antibodies. This can be useful in localization, ELISA, western blots or other immunoanalytical methods.


'"/>


(Date:2/14/2017)... N.C. , Feb. 14, 2017  Wake Forest ... M.D., as its new chief executive officer (CEO). Freischlag ... CEO John D. McConnell , M.D., who last ... position at the Medical Center, after leading it since ... the full scope of Wake Forest Baptist,s academic health ...
(Date:2/10/2017)... Research and Markets has announced the addition ... and Commercial Aspects" to their offering. ... Biomarkers play an ... for selection of treatment as well for monitoring the results. ... in modern medicine. Biochip/microarray technologies and next generation sequencing are ...
(Date:2/8/2017)... 7, 2017  Aware, Inc. (NASDAQ: AWRE ), ... financial results for its quarter and year ended December 31, ... 2016 was $3.9 million compared to $6.9 million in the ... of 2016 was $0.6 million compared to $2.6 million in ... quarter of 2016 was $0.5 million, or $0.02 per diluted ...
Breaking Biology News(10 mins):
Other biology definition