Navigation Links
Hershey-Chase experiment


The Hershey-Chase experiment was a series of experiments conducted in 1952 by Alfred Hershey and Martha Chase that identified DNA to be the genetic material of phages and, ultimately, of all organisms. A phage is a small virus that infects bacteria. It consists of a protein coat that encloses the genetic material. When a phage infects a bacterium, it inserts its genetic material into the bacterium, while its coat remains outside.


In a first experiment, T2 phages with radioactive 32P-labeled DNA infected bacteria. In a second experiment, T2 phages with radioactive 35S-labeled protein infected bacteria. In both experiments, bacteria were separated from the phage coats by blending followed by centrifugation. In the first experiment, most radioactivity was found in the infected bacteria, while in the second experiment most radioactivity was found in the phage coat. These experiments demonstrated that DNA is the genetic material of phage and that protein does not transmit genetic information.

Hershey shared the 1969 Nobel Prize in Physiology or Medicine for his discoveries concerning the genetic structure of viruses


More detailed description

Hershey and Chase knew that T2 consisted of only DNA and protein, and that they somehow manipulated host cells to produce new phages, but did not know if DNA or protein was responsible.

Hershey and Chase radioactively labeled E.Coli and T2 with 32P (phosphorus isotope) and 35S (sulfur isotope) because they knew DNA contains phosphorus, and protein contains sulfur, but not vice versa.

They infected the 32P E.Coli with 32P T2 and 35S E.Coli with 35S T2, and collected the progeny. The results found that the progeny collected from the 32P E.Coli and 32P T2 contained the 32P isotope, while the 35S strains did not, providing more evidence that DNA was the genetic information that bacteriophages inject into bacteria, not protein.


'"/>


(Date:8/5/2015)... 2015 Transparency Market Research has ... - Global Industry Analysis, Size, Share, Growth, Trends and ... recognition is forecast to reach US$ 2,671.8 Mn by ... surveillance systems by civil and government agencies. This is ... terrorist activities across the globe that would elevate the ...
(Date:8/3/2015)... 3, 2015 Synaptics, Inc. (Nasdaq: SYNA ... announced that members of the executive management team will ... Global Technology Leadership Forum on Tuesday, August 11, 2015 ... held at the Sonnenalp Resort in Vail, ... include forward-looking information. An audio webcast of the event ...
(Date:7/31/2015)... SHENZHEN , Chine, 31 juillet 2015 /PRNewswire/ ... (ICG-10, www.icg-10.org ) sera organisée par le ... Shenzhen en Chine. ... année. Depuis son inauguration en 2006, l,ICG est ... influentes dans le domaine des « omiques » et c,est ...
Breaking Biology News(10 mins):Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 2Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 3Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 4Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 5Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 6La 10e International Conference on Genomics (ICG-10) doit s'ouvrir en octobre 2La 10e International Conference on Genomics (ICG-10) doit s'ouvrir en octobre 3
... Societies for Experimental Biology (FASEB) announces the opening ... Matricellular Proteins in Development, Health, and Disease. ... (ECM) are the basis for multi-cellular life and ... metazoan organisms. Structural ECM components such as collagens, ...
... The Federation of American Societies for Experimental Biology (FASEB) ... Conference (SRC): Ciliate Molecular Biology. The 2013 FASEB ... for researchers who study Ciliates as model organisms, to ... making important contributions to a large number of fields ...
... MD The Federation of American Societies for ... for the Science Research Conference (SRC): Mechanism and ... FASEB Science Research Conference on Mechanism and Regulation ... in Saxton Rivers, VT. The "Vermont Transcription Meeting," ...
Cached Biology News:FASEB SRC announces conference: Matricellular Proteins in Development, Health, and Disease 2
Other biology definitionOther Tags