Navigation Links
Glycolysis


Glycolysis is the initial metabolic pathway of carbohydrate catabolism. The most common and well-known form of glycolysis is the Embden -Meyerhof pathway. The term can be taken to include alternative pathways, such as the Entner-Doudoroff Pathway. However, glycolysis will be used here as a synonym for the Embden-Meyerhof pathway.

Glycolysis is the most universal process by which cells of all types derive energy from sugars. It is not the most efficient, but glycolysis proper is completely anaerobic; that is, oxygen is not required.


Contents

Output

Glycolysis converts one molecule of glucose into two molecules of pyruvate, along with "reducing equivalents" in the form of the coenzyme NADH.

The global reaction of glycolysis is:

Glucose + 2 NAD+ + 2 ADP + 2 Pi → 2 NADH + 2 pyruvate + 2 ATP + 2 H2O + 2 H+

So, for simple fermentations, the metabolism of 1 molecule of glucose has a net yield of 2 molecules of ATP. Cells performing respiration synthesize much more ATP but this is not considered part of glycolysis. Eukaryotic aerobic respiration produces an additional 34 molecules (approximately) of ATP for each glucose molecule oxidized.

Location

In eukaryotes glycolysis takes place within the cytosol of the cell (as opposed to the mitochondria, where reactions more closely connected to aerobic metabolism occur). Glucose gets into the cell through facilitated diffusion. In some tissues, skeletal muscle for instance, insulin stimulates this process.

Follow up

The ultimate fate of the pyruvate and NADH produced in glycolysis depends upon the organism and the conditions, most notably the presence or absence of oxygen or other external electron acceptors.

In fermentation, the pyruvate and NADH are anaerobically metabolized to yield any of a variety of products. For example, the bacteria involved in making yogurt simply reduce the pyruvate to lactic acid, whereas yeast produce ethanol and carbon dioxide.

In aerobic organisms, the pyruvate typically enters the citric acid cycle, and the NADH is ultimately oxidized by oxygen during oxidative phosphorylation. Although human metabolism is primarily aerobic, under anaerobic conditions, for example in over-worked muscles that are starved for oxygen, pyruvate is converted to lactate, as in many microorganisms.

Evolution

Glycolysis is the only metabolic pathway common to nearly all living organisms, suggesting great antiquity; it may have originated with the first prokaryotes, 3.5 billion years ago or more.

Pathway

The first step in glycolysis is phosphorylation of glucose by hexokinase (in liver the most important hexokinase is glucokinase which has slightly different properties than the hexokinases in most other cells). This reaction consumes 1 ATP molecule, but the energy is well spent: although the cell membrane is permeable to glucose because of the presence of glucose transport proteins, it is impermeable to glucose 6-phosphate. Glucose 6-phosphate is then rearranged into fructose 6-phosphate by phosphoglucose isomerase. (Fructose can also enter the glycolytic pathway at this point.)

Phosphofructokinase-1 then consumes 1 ATP to form fructose 1,6-bisphosphate. The energy expenditure in this step is justified in 2 ways: the glycolytic process (up to this step) is now irreversible, and the energy supplied to the molecule allows the ring to be split by aldolase into 2 molecules - dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. (Triosephosphate isomerase converts the molecule of dihydroxyacetone phosphate into a molecule of glyceraldehyde 3-phosphate.) Each molecule of glyceraldehyde 3-phosphate is then oxidized by a molecule of NAD+ in the presence of glyceraldehyde 3-phosphate dehydrogenase, forming 1,3-bisphosphoglycerate.

In the next step, phosphoglycerate kinase generates a molecule of ATP while forming 3-phosphoglycerate. At this step glycolysis has reached the break-even point: 2 molecules of ATP were consumed, and 2 new molecules have been synthesized. This step, one of the two substrate-level phosphorylation steps, requires ADP; thus, when the cell has plenty of ATP (and little ADP) this reaction does not occur. Because ATP decays relatively quickly when it is not metabolized, this is an important regulatory point in the glycolytic pathway. Phosphoglyceromutase then forms 2-phosphoglycerate; enolase then forms phosphoenolpyruvate; and another substrate-level phosphorylation then forms a molecule of pyruvate and a molecule of ATP by means of the enzyme pyruvate kinase. This serves as an additional regulatory step.

After the formation of fructose 1,6 bisphosphate, many of the reactions are energetically unfavorable. The only reactions that are favorable are the 2 substrate-level phosphorylation steps that result in the formation of ATP. These two reactions pull the glycolytic pathway to completion.

Etymology

From Greek glyk meaning sweet and lysis meaning dissolving.

See also

External links


'"/>


(Date:8/27/2014)... of fish began exploring land and evolved into tetrapods ... ancient fish used their fishy bodies and fins in a ... scientific mysteries. , Researchers at McGill University published in ... called Polypterus , to help show what might have ... water. Polypterus is an African fish that can ...
(Date:8/27/2014)... when it only requires nerve cells to rearrange existing ... to generate new patterns, a study of monkeys has ... through recordings of electrical activity of brain cell networks. ... of Health. , "We looked into the brain and ... outside the box," said Aaron Batista, Ph.D., an assistant ...
(Date:8/27/2014)... manipulating neural circuits in the brain of mice, ... memories. The research, led by Howard Hughes Medical ... of Technology (MIT), reveals that the connections between ... information about an experience and the part of ... that experience are malleable. , Altering those ...
Breaking Biology News(10 mins):Walking fish reveal how our ancestors evolved onto land 2Scientists plug into a learning brain 2Scientists plug into a learning brain 3Researchers change the emotional association of memories 2Researchers change the emotional association of memories 3Researchers change the emotional association of memories 4
... the lichen Phylloblastia fortuita , new to the Iberian ... Phylloblastia dispersa , is also a new entry for ... outside the tropics. Foliicolous lichens, symbiosis between fungi ... and their presence in environments such as the Iberian Peninsula, ...
... January 11, 2010 New standards to help ensure ... widely used in infant formulas and a variety of ... Food Chemicals Codex (FCC), an internationally recognized compendium of ... for three nucleotides, present in breast milk and commonly ...
... , REDWOOD CITY, Calif. and NEW YORK, ... identity and authentication solutions, has extended its leadership in ... , a leading developer of point-of-sale (POS) and retail ... America, has integrated U.are.U® fingerprint biometrics from ...
Cached Biology News:A new species of lichen discovered in the Iberian Peninsula 2Quality and safety of infant formulas, functional foods enhanced by new standards 2Quality and safety of infant formulas, functional foods enhanced by new standards 3Retail Anywhere Adds U.are.U Biometrics From DigitalPersona to Flagship Point of Sale Solution 2Retail Anywhere Adds U.are.U Biometrics From DigitalPersona to Flagship Point of Sale Solution 3
Other biology definitionOther Tags