Navigation Links
Chromosome walking


Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome. As the traditional chain termination method does not allow long DNA strands to be sequenced, this method works by dividing the long sequence into several consecutive short ones.

The basic technique is as follows:

  1. A primer that matches the beginning of the DNA to sequence is used to synthesize a short DNA strand complementary to the unknown sequence, starting with the primer (see PCR).
  2. The new short DNA strand is sequenced using the chain termination method.
  3. The end of the sequenced strand is used as a primer for the next part of the long DNA sequence.

That way, the short part of the long DNA that is sequenced keeps "walking" along the sequence. The method can be used to sequence entire chromosomes (thus, chromosome walking). A different method with the same purpose which becomes more popular for large-scale sequencing (e.g., the Human Genome Project) is shotgun sequencing.

See also

  • Shotgun sequencing - an alternative method, using random, rather than consecutive, sub-strands.

'"/>


(Date:12/8/2016)... Dec. 8, 2016 Market Research Future published a half ... global Mobile Biometric Security and Service Market is expected to grow ... Market Highlights: ... , Mobile Biometric Security and ... the increasing need of authentication and security from unwanted cyber threats. ...
(Date:12/7/2016)... According to a new market research report "Emotion Detection and Recognition Market by ... Area, End User, And Region - Global Forecast to 2021", published by MarketsandMarkets, ... to USD 36.07 Billion by 2021, at a Compound Annual Growth Rate (CAGR) ... ... MarketsandMarkets Logo ...
(Date:12/7/2016)...   Avanade is helping Williams Martini Racing, ... history, exploit biometric data in order to critically analyse ... competitive edge against their rivals after their impressive, record-breaking ... has worked with Williams during the 2016 season to ... rate, breathing rate, temperature and peak acceleration) for key ...
Breaking Biology News(10 mins):
Other biology definition