Vectors

Vectors are DNA used to transfer genes into a host cell.
A vector must be capable of self-replicating inside a cell.
Marker genes can be used to determine if the gene has been taken up.
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Vectors are vehicles for cloning DNA. A vector provides essential sequences for replicating DNA in a host and selection antibiotic markers. Non-essential sequences are deleted to allow room for the cloning of foreign DNA.
Related ...
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vectors Self-replicating DNA molecules that can be joined with DNA fragments to form recombinant DNA molecules.
veins Thin-walled vessels that carry blood to the heart. Units of the circulatory system that carry blood to the heart.
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Vectors
Figure 3: Comparison of non-integrating plasmids (top) and episomes (bottom). 1 Chromosomal DNA. 2 Plasmids. 3 Cell division. 4 Chromosomal DNA with integrated plasmids ...
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Vectors in gene therapy
Viruses attack their hosts to insert their genetic material into the genetic material of the host. This genetic material contains instructions to produce these viruses.
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vectors: the carriers of DNA genes to be inserted into cells.
veins: channels through which fluid flows toward the heart.
vena cava :the major vein in the human heart; pumps oxygen-poor blood into the right atrium.
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Vectors and Biochemistry for Sequencing by Nested Deletions
John J. Dunn and Matthew Randesi
Biology Department, Brookhaven National Laboratory, Upton, New York 11973
jdunn@bnl.gov ...
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Insect Vectors
Insects provide a system that can deliver pathogens directly to the bloodstream and are essential to the spread of some infections.
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The unit vectors i, j, and k from the given orthogonal coordinate system satisfy the following equalities:
i j = k j k = i k i = j ...
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The early vectors used for gene insertion could, and did, place the gene (from one to 200 copies of it) anywhere in the genome.
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Altered forms are used as vectors for cloning DNA. Bacteriostat. A class of antibiotics that prevents growth of bacterial cells. Bacterium. A single-celled, microscopic prokaryotic organism: a single cell organism without a distinct nucleus.
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Cosmids -- plasmid vectors designed for cloning large fragments of eukaryotic DNA; the vector is a plasmid into which phage lambda cohesive end sites have been inserted. CpG islands -- areas of multiple CG repeats in DNA.
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coli vectors which are used for cDNA and genomic DNA libraries. Filamentous bacteriophages are used to produce single-stranded DNA for sequencing or in vitro mutagenesis (eg. M13), and as vectors for Phage display expression screening systems ...
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Cloning vector: DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self- replication; ...
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Cosmid
A cloning vector consisting of the phage lambda cos site inserted into a plasmid. Such vectors can be packaged into lambda phage or maintained as plasmids. Cosmids are often used to clone large DNA fragments (up to about 40 kilobases).
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A derivation of ColE1, one of the first plasmid vectors widely used.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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pBR322. A derivation of ColE1, one of the first plasmid vectors widely used. (See Plasmid.)
PCR. See Polymerase chain reaction.
Pedigree. A diagram mapping the genetic history of a par- ticular family.
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MULTICOPY PLASMIDS - Present in bacteria at amounts greater than one per chromosome. Vectors for cloning DNA are usually multicopy; there are sometimes advantages in using a single copy plasmid.
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phage - virus that infects bacteria; altered phage can be used as cloning vectors. (short for bacteriophage - "bacteria eater") ...
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RNA virus that encode the enzyme reverse transcriptase so that their RNA can be transcribed into DNA in the host cell; modified retroviruses are used as vectors to introduce genes (or portions thereof) of interest into eukaryotic cells.
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Vector: The DNA "vehicle" used to carry experimental DNA and to clone it. The vector provides all sequences essential for replicating the test DNA. Typical vectors include plasmids, cosmids, phages and YACs.
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Transposable elements, such as certain maize transposable elements and the P elements of Drosophila melanogaster, are also used in gene tagging.
P elements have also been adapted as vectors for introduction of genes into D. melanogaster.
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