Transfection

Transfection
Methods for delivering DNA/RNA into tissue culture cells, include lipofection, electroporation, and calcium phosphate transfection.
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Transfection
First used for the transformation of prokaryotic cells by protein-free DNA or RNA from viruses. Also the process of genetic transformation in eukaryotic cells.
Related Terms: Transformation ...
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Transfection
The introduction of purified phage DNA into a bacterial cell by transformation or electroporation.
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transfection - incorporation of DNA into a cell by incubation of the two together under appropriate conditions.
translation - process whereby mRNA code is used by the ribosome to synthesize a polypeptide chain (protein) from amino acid precursors.
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TRANSFECTION - The process of introducing foreign DNA (or RNA) into a host organism, usually a eukaryotic cell.
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Transfection The introduction of foreign DNA into a host cell.
See also: cloning vector, gene therapy ...
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Transfection: A method by which foreign DNA may be put into a cultured mammalian cell.
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A gene that upon transfection converts a previously immortalized cell to the malignant phenotype.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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A gene that upon transfection enables a primary cell to grow indefinitely in culture. (See Oncogene.) Incomplete dominance.
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Cloning of any DNA sequence comprises of the following four steps: amplification, ligation, transfection, and screening/selection. Initially, the DNA fragment of interest needs to be amplified (many copies need to be produced).
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Introducing DNA into eukaryotic cells, such as animal cells, is called transfection.
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Transfection A general term to describe the introduction of recombinant or vector DNA into host cells.
Transformation Usually refers to passive uptake of DNA by cells, although in nature some bacterial species have a specific active uptake system.
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When the D segment-flanking signals are of the 12 bp type, then the J segment signal sequence will have a 23 bp spacer, as will that at the VH gene.
RAG1 and RAG2 are purified proteins isolated from lymphoid tissue. Co-transfection of RAG1 and RAG2 ...
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Furthermore, we describe the development of a reliable transfection method for motor neurons based on microinjection, which will be extremely useful for dissecting further the molecular basis of membrane dynamics and axonal transport in these cells.
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