Taq polymerase

Taq polymerase lacks a 3' to 5' exonuclease activity. This makes it impossible for it to check the base it has inserted and remove it if it is incorrect, a process common in higher organisms.
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Taq POLYMERASE - A DNA polymerase which is very stable at high temperatures, isolated from the thermophilic bacterium Thermus aquaticus. Very useful in PCR reactions which must cycle repetitively through high temperatures during the denaturation step.
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Taq polymerase. A heat-stable DNA polymerase isolated from the bacterium Therrnus aquaticus, used in PCR. (See Polymerase.) ...
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Taq polymerase: A DNA polymerase isolated from the bacterium Thermophilis aquaticus and which is very stable to high temperatures. It is used in PCR procedures and high temperature sequencing.
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(See Poly(A) polymerase, Polymerase chain reaction, RNA polymerase, Taq polymerase.) Polymorphisms. Variant forms of a particular gene that occur simultaneously in a population. Polynucleotide. A DNA polymer composed of multiple nucleotides.
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Automated, direct cycle sequencing of purified double-stranded PCR products using Taq polymerase and fluorescently-labeled dideoxynucleotide terminators provides a robust and highly reproducible method to identify DNA sequence variations in ...
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Taq polymerase is widely used in current PCR practice (May 2004). A disadvantage of Taq is that it sometimes makes mistakes when copying DNA, leading to mutations (errors) in the DNA sequence, since it lacks 3'-5' proofreading exonuclease activity.
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The polymerase is called Taq polymerase. It is call Taq because it comes from the organism Thermus aquaticus, a bacteria that lives in thermal vents in the ocean.
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Use of a thermostable DNA polymerase (such as Taq polymerase) allows the reactions to be perfo rmed in a thermocycler that automatically adjusts the temperature to the optimal for each reaction through many cycles.
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It's DNA polymerase (called Taq polymerase) is stable at relatively high temperatures and functions optimally at 70 degrees C. This is important because high temperatures will be used to separate the strands of the double helix.
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