Sticky end

Sticky ends
Digestions of double-stranded DNA by many restriction enzymes (e.g. EcoR I) generate ends with a short single-stranded sequences. Such ends are called sticky ends.
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Sticky end/blunt end
(Redirected from Blunt end)
In biology, sticky end and blunt end are the two possible configurations resulting from the breaking of double-stranded DNA.
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sticky ends Term applied to DNA sequences cut with restriction enzymes where the cuts will bond with each other or with another sequence cut with the same enzyme.
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STICKY END - The terminus of a DNA molecule which has either a 3' or 5' overhang, and which typically results from a cut by a restriction endonuclease.
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Sticky end. A protruding, single-stranded nucleotide se- quence produced when a restriction endonuclease cleaves off center in its recognition sequence.
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Sticky ends: After digestion of a DNA with certain restriction enzymes, the ends left have one strand overhanging the other to form a short (typically 4 nt) single-stranded segment.
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: Also known as sticky end. Overhanging ends of
a double-stranded DNA molecule that are
capable of hybridizing with complementary ends.
Complementary (copy) DNA ...
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DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment.
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Sticky end Short section of single-stranded DNA produced at the end of a double-stranded DNA fragment produced by DNA digestion with certain restriction enzymes.
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These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end.
In this case, both DNA preparations have complementary sticky ends and thus can pair with each other when mixed.
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Transposase makes a staggered cut at the target site producing sticky ends, cuts out the transposon and ligates it into the target site.
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Fragments of DNA that has been cut with restriction enzymes have unpaired nucleotides at the ends called sticky ends. All of the fragments will have the same sticky ends. The sticky ends have complimentary bases, so they could rejoin.
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Linker
A synthetic, double-stranded oligonucleotide used to attach sticky ends to a blunt-ended molecule.
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Some restriction enzymes generate blunt ends, cutting across both strands of DNA. Others generate a staggered cut, producing "sticky ends.
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cohesive ends Two DNA ends in the same DNA molecule, or in different molecules, with short overhanging single-stranded segments that are complementary to one another, facilitating ligation of the ends; also known as sticky ends.
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