Southern blot

Southern blot
a technique for transferring DNA fragments separated by agarose gel electrophoresis to a nitrocellulose filter on which specific DNA fragments can be detected using specific radioactive DNA probes; devised by E. Southern ...
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Southern blot
A technique for detecting specific DNA using a labeled DNA probe. DNA (e.g. genomic DNA) is separated by electrophoresis in agarose gel and transferred onto a membrane.
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Dr. John Carpten, of the National Human Genome Research Institute's Laboratory of Cancer Genetics, defines Southern blot.
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terms:
deoxyribonucleic acid (DNA), hybridization ...
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Southern blotting using an insertion sequence as probe revealed that the sequence is present in multiple copies per genome. These additional sequences were isolated by molecular cloning and characterized by sequencing .
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Southern blotting
Transfer by absorption of DNA fragments separated in electrophoretic gels to membrane filters (nylon or nitrocellulose) for detection of specific base sequences by radiolabeled complementary probes.
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Southern blotting
Main article: Southern blot
The Southern blot is a technique employed to ascertain information about the molecular weight and relative amount of a DNA sequence of interest.
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Southern blots
A method for detecting specific DNA fragments seperated on an agarose gel. DNA fragments are first seperated by electrophoresis through an agarose gel.
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Southern blotting
A hybridization technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.
specialized ...
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Southern Blotting
Electrophoresis separates DNA by size but does not provide any information about the nucleotide sequence in the DNA.
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Southern blotting - technique in which DNA fragments, separated by electrophoresis, are immobilized on a paper sheet; specific molecules are then detected with a labeled nucleic acid probe ...
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SOUTHERN BLOT - DNA is separated by electrophoresis (usually in agarose gels), then transferred to nitrocellulose paper or other suitable solid-phase matrix (e.g., nylon membrane), ...
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Southern blotting Transfer by absorption of DNA fragments separated in electrophoretic gels to membrane filters for detection of specific base sequences by radio-labeled complementary probes.
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Southern blot: The transfer (by absorption) of size-separated (by electrophoresis) DNA fragments to a synthetic membrane whereby the presence and rough size of one particular fragment of DNA can be ascertained by detection of specific base sequences ...
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Southern blotting
Scientists often use southern blots to evaluate DNA extracts from tissue samples (Fig. 7). Southern blotting a type of nucleic acid hybridization test, in which single-stranded DNA from two sources interact.
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Southern blotting. See Southern hybridization. Southern hybridization (Southern blotting).
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Southern Blot DNA blot taken from an electrophoresis gel. The original blot type named after its originator Ed Southern, ...
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Southern blot A DNA hybridization procedure in which specific DNA fragments are detected in a mixture by means of a complementary, labeled nucleic acid probe.
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This autoradiogram (Courtesy of P. Borst; from P.A.M. Michels, et al., Nucleic Acids Research 10:2353, 1982.) shows Southern blots of nuclear DNA from clones of Trypanosoma brucei expressing different variant surface glycoproteins (VSGs).
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Transfer of electrophoretically separated RNA molecules from a gel onto an absorbent sheet, which is then immersed in a labeled probe that will hybridize to an RNA of interest to reveal its presence. See Southern blotting.
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by endonucleases, which only cut where there are specific DNA sequences recognized by the enzymes. The restriction fragments are then separated according to length by agarose gel electrophoresis. The resulting gel may be enhanced by Southern blotting.
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Southern blotting -- a technique for transferring electrophoretically resolved DNA segments from an agarose gel to a nitrocellulose filter paper sheet via capillary action; the DNA segment of interest is probed with a radioactive, ...
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