Reverse genetics

Reverse genetics is an approach to discovering the function of a gene that proceeds in the opposite direction of so called forward genetic screens that are more usual in classical genetics.
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Reverse genetics is an approach to discovering the function of a gene that proceeds oppositely to how such discoveries typically unfold in classical genetics, or in forward genetics.
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Reverse genetics has been applied successfully to
plants [examples];
mice [Link];
C. elegans;
and can also be used with the zebrafish.
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Reverse genetics
An approach where a cloned gene with an unknown function is used to disrupt the corresponding chromosomal gene to examine the resulting phenotype.
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Molecular geneticists often use reverse genetics in which a specific mutation is made in vitro and then introduced into an organism's genome.
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Since the protein product of the defective NF1 gene had not been isolated, investigators used the relatively new approach sometimes referred to as reverse genetics or positional cloning.
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Reverse genetics. Using linkage analysis and polymorphic markers to isolate a disease gene in the absence of a known metabolic defect, then using the DNA sequence of the cloned gene to predict the amino acid sequence of its encoded protein.
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