Restriction site

Restriction site
Shortened term for restriction endonuclease recognition sequence.
Related Terms: Restriction enzyme ...
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Restriction site
A DNA sequence recognized and cleaved by a restriction endonuclease. Restriction sites are often 4 to 6 bp, but some sites are 8 bp or longer.
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restriction site
A specific sequence on a DNA strand that is recognized as a "cut site" by a restriction enzyme.
reticular formation ...
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RESTRICTION SITE - See RECOGNITION SEQUENCE.
RETICULOCYTE LYSATE - A lysate of rabbit reticulocytes, which has been extensively digested with micrococcal nuclease to destroy the reticulocyte mRNAs.
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Restriction site: See Restriction enzyme.
Reverse transcriptase: An enzyme which will make a DNA copy of an RNA template - a DNA-dependant RNA polymerase.
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Restriction site: See Restriction enzyme.
Restriction fragment length polymorphism (RFLP): Variation in the distance between restriction enzyme cleavage sites that exist within a population producing unique DNA fingerprint patterns.
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(1990, PNAS 87:8242) a new YAC vector, pCGS1000, has been constructed with an EcoR 1 cloning site flanked by unique restriction sites and polypurine tag sites for capture by triple helix interaction.
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If a restriction enzyme can be found such that it cuts only one possible allele of a section of DNA (that is, the alternate nucleotide of the SNP causes the restriction site to no longer exist within the section of DNA), ...
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If the region between primers A and B contains a restriction site that is polymorphic, the PCR product can be digested with the enzyme and the size of the digestion products analyzed by gel electrophoresis.
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The distance between the locations cut by restriction enzymes (the restriction sites) varies between individuals: so the length of the fragments varies, and the position of certain gel bands differs between individuals (thus polymorphism).
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A brief sketch of the positions of some restriction sites in a DNA sequence (e.g., a plasmid map). Used primarily for designing and identification of recombinant DNA.
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MHC-linked 21-hydroxylase gene (CYP21), gene conversions within the MHC class II genes in mice and humans, many oncogene translocations (BCL2 for example) are attributed to chi-like sequences at the breakpoint region. It acts like a restriction site ...
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These variations result from rare sequence changes that create or destroy restriction sites in the genome. Retinol-binding protein 21 kDa protein retro- backwards, behind retrovirus An RNA virus containing reverse transcriptase.
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A map showing physical locations on a DNA molecule, such as restriction sites, and sequence-tagged sites. (See Mapping.) Plant Pest Act (PPA). See U.S. Department of Agriculture. Plant Variety Act (PVA). See U.S. Department of Agriculture. Plaque.
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