Restriction enzyme

Restriction enzyme is a class of endonuclease that cut specific double-stranded DNA sequences.
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Endonuclease Restriction site ...
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Restriction enzymes are DNA-cutting enzymes found in bacteria (and harvested from them for use). Because they cut within the molecule, they are often called restriction endonucleases.
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restriction enzyme
an endonuclease that cleaves DNA at specific nucleotide sequences
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...
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Restriction enzyme
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA. The enzyme makes two incisions, one through each of the phosphate backbones of the double helix without damaging the bases.
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Enzymes that recognize a specific sequence of double-stranded DNA and cut the DNA at that site. Restriction enzymes are often referred to as molecular scissors.
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Restriction enzymes were discovered in bacteria. Bacteria use them as a defense mechanism to cut up the DNA of viruses or other bacteria.
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restriction enzymes: catalyze the opening of a DNA molecule at a "restriction" point; many leave dangling ends of DNA molecules at the point where the DNA has been opened.
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Restriction Enzymes: Microscopic Scalpels
Isolated from various bacteria, restriction enzymes recognize short DNA sequences and cut the DNA molecules at those specific sites.
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restriction enzymes A series of enzymes that attach to DNA molecules at speci?c nucleotide sequences and cut both strands of DNA at those sites. A bacterial enzyme that cuts DNA at a specific recognition sequence.
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Restriction enzyme, endonuclease: A protein that recognizes specific, short sequences of DNA and cuts at those sites. See also Restriction enzyme cutting site.
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restriction enzyme
A degradative enzyme that recognizes and cuts up DNA (including that of certain phages) that is foreign to a bacterium.
restriction fragment length polymorphisms (RFLPs) ...
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Restriction enzyme: A class of enzymes ("restriction endonucleases") generally isolated from bacteria, which are able to recognize and cut specific sequences ("restriction sites") in DNA.
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Restriction enzyme cutting site: A specific nucleotide sequence of DNA at which a particular restriction enzyme cuts the DNA. Some sites occur frequently in DNA (e.g., every several hundred base pairs), others much less frequently (rare-cutter; e.g.
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The use of restriction enzymes and then ultimately PCR, all of these methods that underlie the recombinant DNA revolution were critical to being able to make maps of the Y. Initially, our first coherent maps of the Y came together in the 1980s.
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Related Terms: Restriction enzyme
A bacterially derived protein that recognizes specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain over 400 such enzymes that recognize and cut over 100 different DNA sequences.
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DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment.
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Blunt end End of a DNA fragment produced by a restriction enzyme which cuts both strands of DNA at the same point, leaving no single-stranded sections. DNA with this type of end is more difficult to ligate ...
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The vector (which is frequently circular) is linearised by means of restriction enzymes, and incubated with the fragment of interest under appropriate conditions that allow for ligation to occur.
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For this particular combination of restriction enzyme and hybridization probe, the pattern of hybridizing bands shown above was obtained.
The length of the restriction fragments recognized by the probe is dimorphic in this population.
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Restriction enzymes cut DNA wherever their "recognition site" (usually between 4 and 8 bases in length) occurs in the DNA sequence. When there are changes between sequences, a recognition site may appear or be lost.
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Restriction
The cleavage of double-stranded DNA by an endonuclease (restriction enzyme). The restriction enzyme distinguishes between self and foreign DNA based upon the modification of its DNA-binding site (for example, by methylation).
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After the discovery of the genetic code and such tools of cloning as restriction enzymes, the avenues of investigation open to geneticists were greatly broadened.
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Often due to the presence of a restriction enzyme cleavage site at one place in the genome in one individual and the absence of that specific site in another individual. See also variable-number-of-tandem-repeats (VNTR) locus.
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Labs (CEPRAP): Electrophoresis, Restriction Enzymes, Bacterial Transformation
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restriction endonuclease - aka restriction enzyme - bacterial enzyme that cuts double-stranded DNA at a specific sequence.
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Restriction fragment length polymorphisms (RFLP) are variation in DNA fragment banding patterns of electrophoresed restriction digests of DNA from different individuals of a species. Often due to the presence of a restriction enzyme cleavage site at ...
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ENDONUCLEASE - Cleaves bonds within a nucleic acid chain; they may b especific for RNA or for single-stranded or double-stranded DNA. A restriction enzyme is a type of endonuclease.
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restriction map - diagrammatic representation of a DNA molecule indicating the sites of cleavage by various restriction enzymes ...
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Usually generated by the action of restriction enzymes. cointegrate An intermediate in the migration of certain DNA transposons in which the donor DNA and target DNA are covalently attached.
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