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Phagemid


Phagemid
A plasmid that contains a portion of a phage genome. Upon co-infection of the host with a Helper phage, the plasmid can be packaged into phage particles. A commonly used type of phagemid is packaged as ssDNA into phage M13 particles.
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Phagemid: A type of plasmid which carries within its sequence a bacteriophage replication origin. When the host bacterium is infected with "helper" phage, the phagemid is replicated along with the phage DNA and packaged into phage capsids.
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Phagemid: A type of plasmid which carries within its sequence a bacteriophage replication origin (ori). When the host bacterium is infected with "helper" phage, the phagemid is replicated along with the phage DNA and packaged into phage capsids.
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The standard phagemid will have been produced in large quantity, purified, and stored as individual aliquots kept at -70 C.
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Phagemid A plasmid-based vector which carries a filamentous phage ori , leading to production of single-stranded copies of the phagemid when the cell is infected with the relevant helper phage.
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cloning vector - intentionally designed artificial DNA construct used by molecular biologists to amplify selected pieces of DNA inserted into the construct; examples include plasmid, phage, phagemid, cosmid, fosmid, ...
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