Overlapping clones

Overlapping clones See: genomic library
Updated 26-Aug-06
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Overlapping clones: See genomic library.
Package: In recombinant DNA procedures, refers to the step of incorporation of cosmid or other lambda vector DNA with an insert into a phage head for transduction of DNA into host.
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Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene. Clone.
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A genetic map depicting the relative order of a linked (contiguous) library of small overlapping clones representing a complete chromosomal segment.
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Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.)
Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene.
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Contig maps are important because they provide the ability to study a complete, and often large segment of the genome by examining a series of overlapping clones which then provide an unbroken succession of information about that region.
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A restriction fragment isolated from the end of the positive clones is used to reprobe the genomic library for overlapping clones. This process is repeated several times to walk across the chromosome and reach the gene of interest.
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