Overhang

Overhang: A terminus of a duplex DNA molecule which has one or more unpaired nucleotides in one of the two strands (hence either a 3' or 5' overhang). Cleavage of DNA with many restriction endonucleases leaves such overhangs (see Sticky End).
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The overhangs thus produced can still hybridize ("anneal") with each other, even if they came from different parent DNA molecules, and the enzyme ligase will then covalently link the strands.
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Homopolymer tailing Extension of the 3' ends of a piece of double-stranded DNA using the enzyme terminal transferase and a single deoxynucleoside triphosphate as substrate, resulting in a 3' overhang composed of a single base repeat.
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The fragment ends are then blunted by treatment with Mung Bean nuclease and T4 DNA polymerase, and are ligated to the blunt-end side of an adapter which has a 3' overhang (ACAC) at the other end.
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Because recognition sequences differ between restriction enzymes, the length and the exact sequence of a sticky-end "overhang", as well as whether it is the 5' or the 3' strand that overhangs, depends on which enzyme produced it.
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Overhang
PAGE See Polyacrylamide Gel Electrophoresis (PAGE)
PCR See Polymerase chain reaction (PCR)
PFGE See Pulsed-Field Gel Electrophoresis (PFGE)
Phenotype
Phosphatase
Polyacrylamide Gel Electrophoresis (PAGE)
Polymerase Chain Reaction (PCR) ...
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The ends of the cut have an overhanging piece of single-stranded DNA.
These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end.
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: Also known as sticky end. Overhanging ends of
a double-stranded DNA molecule that are
capable of hybridizing with complementary ends.
Complementary (copy) DNA ...
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The end of a tRNA molecule to which an amino acid becomes bound. It contains both the 5' and 3' ends of the tRNA. The 3'-terminal sequence of cytidine-cytidine-adenosine (CCA) overhangs the end, ...
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cohesive ends Two DNA ends in the same DNA molecule, or in different molecules, with short overhanging single-stranded segments that are complementary to one another, facilitating ligation of the ends; also known as sticky ends.
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