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Northern hybridization


Northern hybridization. (Northern blotting). A procedure in which RNA fragments are transferred from an agarose gel to a nitrocellulose filter, where the RNA is then hybridized to a radioactive probe. (See Hybridization.) ...
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(See Northern hybridization, Southern hybridization.) Hybridoma. A hybrid cell, composed of a B Iymphocyte fused to a tumor cell, which grows indefinitely in tissue culture and is selected for the secretion of a specific antibody of interest.
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Exons and cDNAs are being assessed for expression by performing RT-PCR and Northern hybridization using RNA isolated from appropriate breast cancer cell lines and primary tumors.
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