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Nick translation


Nick translation
A method which uses DNA polymerase I to first produce a nick in a DNA duplex, then degrade stretch of single-stranded DNA using its 5'-exonuclease while synthesizing a new strand in its place.
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Nick translation
Method used to introduce 32p into a DNA probe so that the probe can be detected.
Related Terms: Deoxyribonucleic acid (DNA) ...
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NICK TRANSLATION - A method for introducing labeled nucleotides into a double-stranded DNA molecule which involves making small nicks in one strand with DNase, and then repairing with DNA polymerase I.
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Nick translation: A method for incorporating radioactive isotopes (typically 32P) into a piece of DNA. The DNA is randomly nicked by DNase I, and then starting from those nicks DNA polymerase I digests and then replaces a stretch of DNA.
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Nick translation. A procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of radioactive nucleotides from the nicked sites by DNA polymerase I.
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