Mutagenesis

The production of either random or specific mutations in a piece of cloned DNA. Typically, the DNA will then be reintroduced into a cell or an organism to assess the results of the mutagenesis.
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Mutagenesis is a way of making changes to the sequence of nucleotides in the DNA.
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Mutagenesis (the creation or formation of a mutation) can be used as a powerful genetic tool.
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Mutagenesis
The formation of mutations.
Mutant
An organism with an altered base sequence in one or several genes. Usually refers to an organism with a mutation that causes a phenotypic difference from the wild-type.
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For mutagenesis, we superimpose all possible substitutions of individual target sites upon the previously described mutations. Approximately 200 sites, covering a large portion of the active site, were chosen for saturation mutagenesis.
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Directed mutagenesis
Two new papers examining the phenomena of directed mutations have recently appeared in the literature. I'll quickly review these experiments in the next post.
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Saturation mutagenesis can also be carried out in vitro (kit).
Transposons can also be used in genome sequencing projects.
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Site-Directed Mutagenesis: The introduction of a mutation, usually a point mutation or an insertion, into a particular location in a cloned DNA fragment.
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site-directed mutagenesis A set of methods used to create specific alterations in the sequence of nucleotides in a gene. site-specific recombination A type of genetic recombination that occurs only at specific sequences.
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Pauling first described the a-helix nearly 50 years ago, yet new features of its structure continue to be discovered, using peptide model systems, sitedirected mutagenesis, advances in theory, ...
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Filamentous bacteriophages are used to produce single-stranded DNA for sequencing or in vitro mutagenesis (eg. M13), and as vectors for Phage display expression screening systems ...
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DNA that has been created artificially. Basic recombinant DNA techniques include restriction digestion, ligation, site-directed mutagenesis, PCR, and cloning in plasmid or other vectors.
Other Resources
PubMed Google ...
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Site-directed mutagenesis. The process of introducing spe- cific base-pair mutations into a gene. Small nuclear RNA (snRNA).
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