Megabase Sequencing of
Human Immune
Receptor LociLeroy E. Hood
Department of Molecular
Biotechnology; University of Washington; Seattle, WA 98195
206/685-7367, Fax: -7301 ...
Full article >>>Megabase cloning. The
cloning of very large
DNA fragments. (See
Cloning.) ...
Full article >>>Megabase (Mb): Unit of length for
DNA fragments equal to 1 million
nucleotides and roughly equal to 1 cM.
Melting: The dissociation of a duplex
nucleic acid molecule into single
strands, usually by increasing temperature. See
Denaturation.
Full article >>>See
megabase.
Related Terms:
Megabase (Mb)
Unit of length for
DNA fragments equal to 1 million
nucleotides and roughly equal to 1 cM.
Full article >>>(See
Directional cloning,
Megabase cloning, Molecular
cloning,
Subcloning.)
Coat protein (
capsid). The coating of a
protein that enclosed the
nucleic acid core of a
virus.
Codon.
Full article >>>The
genome is about 43
megabases
long and includes approximately 10,000 genes. Currently there is a project underway to produce mutant knockouts for every
gene in the
genome of N. crassa.
Full article >>>As a very rough rule of thumb, we shall assume that 1 cM on a
chromosome encompasses 1
megabase (1 Mb = 106 bp) of
DNA. But for the reasons mentioned above, this relationship is only approximate.
Full article >>>In the 24 April 2003 issue of Nature, the
genome of N. crassa was reported as completely
sequenced. The
genome is about 43
megabases
long and includes approximately 10,000 genes.
Full article >>>Agarose gels: A
polysaccharide gel used to measure the size of
nucleic acids (in
bases or
base pairs). See "
Gel Electrophoresis". This is the
gel of choice for
DNA or
RNA in the range of thousands of
bases in length, or even up to 1
megabase if you ...
Full article >>>'"/>