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Ligation


Ligation
Enzyme catalyzed joining of two DNA fragments. The two DNA fragments must have compatible ends and at least one of the ends must have a 3' phosphate group.
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Ligation
The process of joining two or more DNA fragments together.
Related Terms: Deoxyribonucleic acid (DNA) ...
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Ligation
The formation of a phosphodiester bond between two adjacent bases separated by a single-strand break. Catalyzed by DNA ligase.
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Ligation Possibilities
If you remove the two restriction enzymes and provide the conditions for DNA ligase to do its work, the pieces of these plasmids can rejoin (thanks to the complementarity of their sticky ends).
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LIGATION - See DNA LIGASE.
LINEAR EPITOPE - An epitope formed by a series of amino acids which are adjacent to each other within the primary structure of the protein.
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Ligation: The process of splicing two pieces of DNA together. In practice, a pool of DNA fragments are treated with ligase (see "Ligase") in the presence of ATP, and all possible splicing products are produced, ...
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tubal ligation A contraceptive procedure in women in which the oviducts are cut, preventing the ova from reaching the uterus.
tubal pregnancy Occurs when the morula remains in the oviduct and does not descend into the uterus.
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Unambiguous ligation requires that alternative complexes with perfect base pairing not be possible with the combination of hexamers used.
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Ethics -- the study of fundamental principles which defines values and determines moral duty and obligation. Erythrocytes -- the hemoglobin-containing cell found in the blood of vertebrates.
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Ligation In vitro reaction used to make the covalent phosphodiester linkage between two double stranded DNA ends.
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Cloning of any DNA sequence comprises of the following four steps: amplification, ligation, transfection, and screening/selection. Initially, the DNA fragment of interest needs to be amplified (many copies need to be produced).
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And I think that the case could be made that it is your obligation to allow the information about you to be used so your children and their children will have access to an equally good healthcare system in the future.
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RNA ligation is required to reform circles.
Reverse genetics studies of several such RNAs identified a secondary structure element, dubbed the hammerhead as responsible for the self-cleavage of the RNA.
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This should not be surprising; as discussed above, evolution as a problem-solving strategy is under no obligation to find the single best solution, merely one that is good enough.
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January 1 - (Roman Catholic Church) a holy day of obligation
Synonyms: Solemnity of Mary
3.
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While the genetic approach is not new (domestication, plant traditional selection methods), progress made in the genetic field in the past 20 years lead to the obligation to tighten laws.
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cohesive ends Two DNA ends in the same DNA molecule, or in different molecules, with short overhanging single-stranded segments that are complementary to one another, facilitating ligation of the ends; also known as sticky ends.
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