Ligase

Ligase
Enzyme that catalysis the joining of two DNA fragments with compatible ends. Ligase is required for DNA replication and recombination. Ligase used for in vitro ligation is usually purified from bacteria.
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Definition of ligase :
An enzyme that can rejoin a broken phosphodiester bond in a nucleic acid.
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LCR uses both a DNA polymerase enzyme and a DNA ligase enzyme to drive the reaction. Like PCR, LCR requires a thermal cycler to drive the rxn and each cycle results in a doubling of the target nucleic acid molecule.
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DNA ligase catalyzes the formation of the covalent bonds between the Okazaki fragments.
Replication Forks
DNA synthesis occurs at numerous different locations on the same DNA molecule (hundreds in a human chromosome).
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Ligase chain reaction is an amplification method which involves the ligation of two sets of adjacent oligonucleotides.
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Ligase (DNA ligase)
An enzyme that repairs single-stranded discontinuities in double-stranded DNA molecules in the cell. Ligase joins a 3'-OH residue of a deoxyribonucleotide to the 5'-phosphate residue of an adjacent deoxyribonucleotide.
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LIGASE - See DNA LIGASE.
LIGATION - See DNA LIGASE.
LINEAR EPITOPE - An epitope formed by a series of amino acids which are adjacent to each other within the primary structure of the protein.
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Ligase (DNA ligase). An enzyme that catalyzes a condensation reaction that links two DNA molecules via the formation of a phosphodiester bond between the 3' hydroxyl and 5' phosphate of adjacent nucleotides.
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Ligase: An enzyme, T4 DNA ligase, which can link pieces of DNA together. The pieces must have compatible ends (both of them blunt, or else mutually compatible sticky ends), and the ligation reaction requires ATP.
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DNA ligase In recombinant DNA technology, an enzyme that seals together two DNA fragments from different sources to form a recombinant DNA molecule.
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DNA ligase
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A linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.
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DNA ligase - fills in nicks and gaps made in polynucleotide strands
DNA renaturation (hybridization) - process whereby two complementary nucleic acid strands form a double helix during an annealing period; ...
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DNA ligase - protein that joins (ligates) DNA strands; used by cells for DNA repair, by molecular biologists for gene cloning.
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Sealed with DNA ligase, these molecules are functioning plasmids that are capable of conferring resistance to both ampicillin and kanamycin. They are molecules of recombinant DNA.
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DNA ligase. See Ligase. DNA polymerase. See Polymerase. DNA polymorphism. One of two or more alternate forms (alleles) of a chromosomal locus that differ in nucleotide sequence or have variable numbers of repeated nucleotide units.
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The reaction is catalysed by the enzyme DNA ligase.
Lysogen Bacterial cell or strain which carries a copy of a bacteriophage genome in its chromosome. Genes within the prophage will be expressed providing their promoters are active.
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Through the cascade of E1 ubiquitin activating, E2 ubiquitin conjugating, and E3 ubiquitin ligase enzymes, ubiquitin monomers are attached sequentially to target proteins.
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The chemical bonds that the enzymes cleave can be reformed by other enzymes known as ligases, so that restriction fragments carved from different chromosomes or genes can be spliced together, provided their ends are complementary (more below).
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Okazaki fragments: new sections of DNA that are placed along the lagging strand during DNA replication and are joined together by DNA ligase to produce a new DNA strand.
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In mammals, the CNC family members nuclear factor-erythroid 2 p45-related factors 1 and 2 (Nrf1 and Nrf2) have been shown to be involved in the transcriptional up-regulation of cytoprotective genes including those encoding glutamate cysteine ligase, ...
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Daniel Nathans and Hamilton Smith received the 1978 Nobel Prize in physiology or medicine for their isolation of restriction endonucleases, which are able to cut DNA at specific sites. Together with ligase, which can join together fragments of DNA, ...
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Lligase -- an enzyme that functions in DNA repair. Linkage -- the greater association in inheritance of two or more nonallelic genes than is to be expected from independent assortment; genes are linked because they reside on the same chromosome.
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DNA ligase An enzyme that creates a phosphodiester bond between the 3' end of one DNA segment and the 5' end of another. DNA polymerase An enzyme that catalyzes template-dependent synthesis of DNA from its dNTP precursors.
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