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Gel electrophoresis


Pulsed-Field Gel Electrophoresis (PFGE)
An electrophoresis technique for separation of large DNA. Alternating electrical field is applied to run DNA through a low density agarose gel matrix.
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Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point.
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Gel electrophoresis
The process of separating charged species by subjecting them to a voltage gradient. A gel (made of agarose or polyacrylamide) provides mechanical support and prevents mixing of the molecules being separated.
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Gel electrophoresis See: electrophoresis
Gene The fundamental physical and functional unit of heredity.
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Gel electrophoresis is an application of electrophoresis in molecular biology. The content of the buffers (solutions) and gels used to enhance viscosity greatly affects the mobility of micromolecules.
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gel electrophoresis
(jell eh-lek-troh-for-ee-sis)
The separation of nucleic acids or proteins, on the basis of their size and electrical charge, by measuring their rate of movement through an electrical field in a gel.
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2D Gel Electrophoresis to Identify Cellular Proteins
While computer-based methods are powerful, they can only predict the function of proteins for which some information is already available.
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AGAROSE GEL ELECTROPHORESIS - A method for separating nucleic acids (DNA or RNA) within a gel made of agarose in a suitable buffer under the influence of an electrical field.
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Agarose gel electrophoresis: A method to analyze the size of DNA (or RNA) fragments. In the presence of an electric field, larger fragments of DNA move through a gel slower than smaller ones, producing different migrating "bands".
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Animation of Gel Electrophoresis
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Denaturing gradient gel electrophoresis (DGGE)
A method for separating DNA fragments according to their mobilities under increasingly denaturing conditions (usually increasing formamide/urea concentrations).
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Agarose gel electrophoresis. A matrix composed of a highly purified form of agar that is used to separate larger DNA and RNA molecules ranging 20,000 nucleotides. (See Electrophoresis.) Alleles.
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gel electrophoresis the process by which nucleic acids (DNA or RNA) or proteins are separated by size according to movement of the charged molecules in an electrical field.
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a technique for transferring DNA fragments separated by agarose gel electrophoresis to a nitrocellulose filter on which specific DNA fragments can be detected using specific radioactive DNA probes; devised by E. Southern ...
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If the region between primers A and B contains a restriction site that is polymorphic, the PCR product can be digested with the enzyme and the size of the digestion products analyzed by gel electrophoresis.
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The DNA is digested using restriction enzymes and separated using gel electrophoresis as described above.
The fragments on the gelatin are transferred to a filter by blotting.
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The gel can be made so that its pores are just the right dimensions for separating molecules within a specific range of sizes and shapes. Smaller fragments usually travel further than large ones. The process is sometimes called gel electrophoresis.
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