FISH

FISH Mapping on DNA Fibers. The fluorescence microscope reveals several individual cloned DNA fibers from yeast artificial chromosomes (YACs, in blue) after molecular combing to attach and stretch the DNA molecules across a glass microscope slide.
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Thomas Ried defines fluorescence in situ hybridization (FISH).
illustrated:
View illustration ...
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FISH
See fluorescence in situ hybridization.
Related Terms: Fluorescence in situ hybridization (FISH) ...
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FISH using pairs of probes from distant segments of the same yeast chromosome reveals pairing of homologues in diploid interphase nuclei (ref). In a minority of nuclei, the pairing of homologous loci is particularly intimate.
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FISH (fluorescence in situ hybridization): A mapping technique that uses fluorescent tags to identify specific locations of chromosomes.
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FISH
Location of the gene for muscle glycogen phosphorylase on human chromosome 11 ...
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FISH (fluorescence in situ hybridization): A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less- condensed somatic interphase chromatin.
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CGH and FISH is a method for analyzing genomic DNA for unbalanced genetic alterations.
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FISH -- florescent in situ hybridization: a technique for uniquely identifying whole chromosomes or parts of chromosomes using florescent tagged DNA.
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We mapped by FISH five HPV18 integration sites: three on normal chromosomes 8 at 8q24 and two on derivative chromosomes, der(5)t(5;22;8)(q11;q11q13;q24) and der(22)t(8;22)(q24;q13), which have chromosome 8q24 material.
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In this newer technique, several different probes specific of one chromosome pair, carrying different amounts of a set of fluorescent dyes, are hybridized to the chromosomes in a technique known as fluorescent in situ hybridization (FISH).
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FISH permits analysis of proliferating (
metaphase cells
) and non-proliferating (
interphase nuclei
) cells and is useful in determining the percentage of neoplastic cells before and after therapy (minimal residual disease) (see examples at ...
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