Electroporation A process using high-voltage current to make
cell membranes permeable to allow the introduction of new
DNA; commonly used in
recombinant DNA technology.
See also:
transfection ...
Full article >>>Electroporation A method for
transferring
DNA (or other small
molecules) into
cells by exposure to a rapid pulse of high voltage, which causes the
transient formation of small pores in the
cell membrane.
Full article >>>Electroporation is another way to make holes in
cells, by briefly shocking them with an electric field of 100-200V. Now
plasmid DNA can enter the
cell through these holes. Natural membrane-repair mechanisms will close these holes afterwards.
Full article >>>Electroporation involves using an electric current to create pores in the
cell wall and
plasma membrane for
DNA to enter.
Full article >>>Electroporation: A method for introducing foreign
nucleic acid into
bacterial or eukaryotic
cells that uses a brief, high voltage DC charge which renders the
cells permeable to the
nucleic acid.
Full article >>>(See
Agarose gell
electrophoresis, Polycrylamide gell
electrophoresis.)
Electroporation.
Full article >>>This is done via
electroporation (using electricity to
transfer the
DNA across the
cell membrane).
Full article >>>Methods for delivering
DNA/
RNA into
tissue culture cells, include lipofection,
electroporation, and calcium phosphate
transfection.
Related
Stable transfection Transient transfection ...
Full article >>>The
cells are then briefly heat shocked so the
plasmid can cross the
plasma membrane. An alternate method,
electroporation, uses a short electrical pulse to open pores in the
plasma membrane, allowing the
plasmid to pass through.
Full article >>>'"/>