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Electroporation


Electroporation A process using high-voltage current to make cell membranes permeable to allow the introduction of new DNA; commonly used in recombinant DNA technology.
See also: transfection ...
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Electroporation
A method for transferring DNA (or other small molecules) into cells by exposure to a rapid pulse of high voltage, which causes the transient formation of small pores in the cell membrane.
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Electroporation is another way to make holes in cells, by briefly shocking them with an electric field of 100-200V. Now plasmid DNA can enter the cell through these holes. Natural membrane-repair mechanisms will close these holes afterwards.
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Electroporation involves using an electric current to create pores in the cell wall and plasma membrane for DNA to enter.
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Electroporation: A method for introducing foreign nucleic acid into bacterial or eukaryotic cells that uses a brief, high voltage DC charge which renders the cells permeable to the nucleic acid.
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(See Agarose gell electrophoresis, Polycrylamide gell electrophoresis.) Electroporation.
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This is done via electroporation (using electricity to transfer the DNA across the cell membrane).
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Methods for delivering DNA/RNA into tissue culture cells, include lipofection, electroporation, and calcium phosphate transfection.
Related
Stable transfection Transient transfection ...
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The cells are then briefly heat shocked so the plasmid can cross the plasma membrane. An alternate method, electroporation, uses a short electrical pulse to open pores in the plasma membrane, allowing the plasmid to pass through.
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