DNA ligase

DNA ligase catalyzes the formation of the covalent bonds between the Okazaki fragments.
Replication Forks
DNA synthesis occurs at numerous different locations on the same DNA molecule (hundreds in a human chromosome).
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DNA ligase
The enzyme that joins the 5' and 3' ends of polynucleotide chains by the formation of a phosphodiester bond between them.
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DNA ligase In recombinant DNA technology, an enzyme that seals together two DNA fragments from different sources to form a recombinant DNA molecule.
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DNA ligase
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A linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain.
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DNA LIGASE - An enzyme (usually from the T4 bacteriophage) which catalyzes formation of a phosphodiester bond between two adjacent bases from double-stranded DNA fragments. RNA ligases also exist, but are rarely used in molecular biology.
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DNA ligase - fills in nicks and gaps made in polynucleotide strands
DNA renaturation (hybridization) - process whereby two complementary nucleic acid strands form a double helix during an annealing period; ...
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DNA ligase - protein that joins (ligates) DNA strands; used by cells for DNA repair, by molecular biologists for gene cloning.
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DNA ligase: Enzymatic activity responsible for creating phosphodiester bonds between the 5' end of one strand of DNA and the 3' end of another strand. Requires the presence of a 5' phosphate on one strand, and a 3' Hydroxyl group on the second strand.
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Sealed with DNA ligase, these molecules are functioning plasmids that are capable of conferring resistance to both ampicillin and kanamycin. They are molecules of recombinant DNA.
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DNA ligase. See Ligase. DNA polymerase. See Polymerase. DNA polymorphism. One of two or more alternate forms (alleles) of a chromosomal locus that differ in nucleotide sequence or have variable numbers of repeated nucleotide units.
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The reaction is catalysed by the enzyme DNA ligase.
Lysogen Bacterial cell or strain which carries a copy of a bacteriophage genome in its chromosome. Genes within the prophage will be expressed providing their promoters are active.
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Use of a thermostable DNA ligase allows the reaction to be cycled in conventional thermocyclers. The specificity of the reaction is such that only oligonucleotides which hybridize without mismatch at their junction are ligated.
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Eventually, the last RNA attaches, and DNA polymerase and DNA ligase come along to convert the RNA (of the primers) to DNA, and seal the gaps in between the Okazaki fragments.
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The two fragments may then be covalently bonded by DNA ligase. Blunt ends may be ligated, but the reaction is significantly slower. These terms are most commonly used to describe the product of a restriction enzyme digestion of DNA.
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Ligase: An enzyme, T4 DNA ligase, which can link pieces of DNA together. The pieces must have compatible ends (both of them blunt, or else mutually compatible sticky ends), and the ligation reaction requires ATP.
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The ligating or attaching of blunt-ended pieces of DNA by T4 DNA ligase. Used in creating hybrid vectors.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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Okazaki fragments: new sections of DNA that are placed along the lagging strand during DNA replication and are joined together by DNA ligase to produce a new DNA strand.
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DNA replication for the lagging strand is discontinuous and away from the replication fork. The small fragments synthesized are called Okazaki fragments and later stitched together by DNA Ligase.
Related ...
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DNA ligase An enzyme that creates a phosphodiester bond between the 3' end of one DNA segment and the 5' end of another. DNA polymerase An enzyme that catalyzes template-dependent synthesis of DNA from its dNTP precursors.
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