Denature

Denature
To provoke structural changes in a molecule which disrupt its biological activity. In DNA it refers to the separation of the two component strands caused by the breaking of the hydrogen bonds.
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denatured enzymes lose their catalytic power
denatured antibodies can no longer bind antigen
A mutation in the gene encoding a protein is a frequent cause of altered tertiary structure.
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denature To change the configuration of a protein molecule such that it loses specificity and no longer functions as an enzyme.
dendrite Any of nerve cell processes that conduct impulses toward the cell body.
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Denatured alcohol
Main article: Denatured alcohol
In most jurisdictions, the sale of ethanol, as a pure substance or in the form of alcoholic beverages, is heavily taxed.
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Denature the process whereby a protein looses its native conformation
(de = from down out; nata = born produced)
Dendrite the "incoming" end of a neuron
(dendro = tree) ...
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Denature. To induce structural alterations that disrupt the biological activity of a molecule.
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It involves using a thermostable DNA Polymerase such as Taq (Thermus aquaticus) and repeatedly heating to denature strands of DNA, followed by cooling to allow polymerization of new strands.
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An in situ methodology employing solution conditions has been developed for binding oligodeoxyribonucleotide 'third-strands' to chromosomal DNA targets in non-denatured protein-depleted metaphase spreads and interphase nuclei.
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Proteins therefore, are ususally denatured in the presense of a detergent such as sodium dodecyl sulfate/sodium docecyl phosphate (SDS/SDP) that coats the proteins with a negative charge.
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After electrophoresis, the DNA in the gel is denatured by soaking the gel in an alkaline solution. Then a nitrocellulose or nylon membrane is layed on top of the agarose gel and absorbant paper is layered on top of the membrane.
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At the temperature DNA denatures (strands separate) proteins from most organisms (like DNA polymerases) also come apart. This presents a problem.
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heteroduplex analysis: denatured suspected mutant DNA is annealed with denatured reference DNA; mismatches are detected by altered mobility in electrophoresis.
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Denaturing gradient gel electrophoresis (DGGE): Resolves partially denatured double stranded DNA in precisely conditions of temperature and denaturant concentration.
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The cells are lysed and the DNA is denatured by treating it with an alkaline solution. When the probe is mixed with the denatured DNA, it will form a double-helix in the area where the gene has complimentary bases.
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Tm - The midpoint of the temperature range over which DNA is melted or denatured by heat; the temperature at which a duplex nucleic acid molecule is 50% melted into single strands, ...
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Changes in pH will also denature the enzyme by changing the shape of the enzyme. Enzymes are also adapted to operate at a specific pH or pH range.
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Random primed synthesis: If you have a DNA clone and you want to produce radioactive copies of it, one way is to denature it (separate the strands), then hybridize to that template a mixture of all possible 6-mer oligonucleotides.
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Some archaea produce unusually high concentrations of thermoprotective proteins (heat shock proteins), which are found in all cells. These proteins help refold partially denatured proteins.
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