Cloning vector

Cloning Vector. Despite the advantages of certain lambda vectors to preferentially clone longer cDNAs, plasmids are considered advantageous given the ease of subsequent manipulation, sequence generation, ...
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Cloning vector
A DNA molecule that is capable of replication in a suitable host cell, that has suitable site(s) for the insertion of DNA fragments by recombinant DNA techniques, ...
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cloning vector - intentionally designed artificial DNA construct used by molecular biologists to amplify selected pieces of DNA inserted into the construct; examples include plasmid, phage, phagemid, cosmid, fosmid, ...
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cloning vector
An agent used to transfer DNA in genetic engineering, such as a plasmid that moves recombinant DNA from a test tube back into a cell, or a virus that transfers recombinant DNA by infection.
closed circulatory system ...
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Cloning vector: DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self- replication; ...
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BAC is a cloning vector propagated as a mini-chromosome in a bacteria host. Inserts of 100-300 kb can be cloned in BAC vectors.
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Vector ...
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To increase the number of copies of a DNA sequence, in vivo by inserting into a cloning vector that replicates within a host cell, or in vitro by polymerase chain reaction (PCR). Anaerobe. An organism that grows in the absence of oxygen. See Aerobe.
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Cloned DNA -- any DNA fragment that passively replicates in the host organism after it has been joined to a cloning vector. Codon -- a sequence of three nucleotides in mRNA that specifies an amino acid. Consanguinity -- genetic relationship.
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Phages play an important role in molecular biology as cloning vectors to insert DNA into bacteria.
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BAC: Bacterial Artificial Chromosome " a cloning vector capable of carrying between 100 and 300 kilobases of target sequence. They are propagated as a mini-chromosome in a bacterial host.
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To do this, a region on the chromosome is identified and DNA homologous to that region is engineered into a cloning vector.
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A short DNA sequence containing several re-striction enzyme recognition sites that is contained in cloning vectors.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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In molecular genetics refers to a DNA sequence of interest that has been inserted into a cloning vector such as a plasmid or bacteriophage.
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Modern cloning vectors include selectable antibiotic resistance markers, which allow only for cells in which the vector has been transfected to grow. However this selection step does not guarantee that the DNA insert is present in the vector.
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cosmid A cloning vector, used for cloning large DNA fragments; generally contains segments derived from bacteriophages and various plasmids. cotransport The simultaneous transport, by a single transporter, of two solutes across a membrane.
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