CDNA library

Dr. Eric Green, of the National Human Genome Research Institute's Genome Technology Branch, defines cDNA library.
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terms:
cloning, deoxyribonucleic acid (DNA), gene, genome, messenger RNA (mRNA), protein, ribonucleic acid (RNA), amino acids ...
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cDNA library
A library composed of complementary copies of cellular mRNAs. See library.
Related Terms: Complementary DNA (cDNA) ...
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cDNA Library Analysis
Projects to sequence, map, and further analyze random cDNAs are ongoing. In sequencing >600 clones from a human testes cDNA library, Michael Jones (Cambridge University) found that 3% were identical to known genes and 70% novel.
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cDNA LIBRARY - A collection of cDNA fragments, each of which has been cloned into a separate vector molecule.
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cDNA library. A library composed of complementary copies of cellular mRNAs. (See Library.)
Cellular oncogene (proto-oncogene). A normal gene that when mutated or improperly expressed contributes to the development of cancer. (See Oncogene.) ...
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A cDNA library is usually just a mixture of bacteria, where each bacteria carries a different plasmid.
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cDNA library. A library composed of complementary copies of cellular mRNAs. (See Library.) Cellular oncogene (proto-oncogene). A normal gene that when mutated or improperly expressed contributes to the development of cancer.
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Thus a genomic library contains all of the sequences present in the genome and a cDNA library contains copies of all the mRNA molecules present in the extracted cells.
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sequencing several hundred base pairs from both ends of cDNA clones taken from a cDNA library), and the resulting sequence is a relatively low quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides.
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A cDNA library that only contains those cDNAs that are expressed in a particular type of cell or tissue or under particular growth conditions. The cDNA common to all conditions is removed by hybridization with excess RNA from other cells (i.e.
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A unique DNA sequence derived from a cDNA library (therefore from a sequence which has been transcribed in some tissue or at some stage of development).
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For example, PCR can be used to introduce restriction enzyme sites, or to mutate (change) particular bases of DNA. PCR can also be used to determine whether a particular DNA fragment is found in a cDNA library.
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