Amplification

PCR (in vitro DNA amplification)
Described as being to genes what Gutenberg's printing press was to the written word, ...
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Some organisms have evolved mechanisms of gene amplification to provide gene products needed in large quantities. These mechanisms include:
Elaboration of small "extrachromosomal" units that replicate to high copy number (rDNA); ...
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A target nucleic acid amplification method that uses RNA transcription (RNA polymerase) and DNA synthesis (reverse transcriptase) to produce RNA amplicon from a target nucleic acid. TMA can be used to target both RNA and DNA.
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Dr. Lawrence Brody, of the National Human Genome Research Institute's Genetic and Molecular Biology Branch, defines gene amplification.
related
terms:
cell, deoxyribonucleic acid (DNA), gene, oncogene ...
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Amplification of microsatellites
Microsatellites can be amplified for identification using Polymerase Chain Reaction (PCR), using templates of flanking regions (primers).
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AMPLIFICATION - Refers to the production of additional copies of a chromosomal sequence, found either as intrachromosomal or extrachromosomal DNA. Also refers to the in vitro process in the polymerase chain reaction.
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Amplification
An increase in the number of copies of a specific DNA fragment; can be in vivo (eg amplification of a repeat sequence as in fragile X syndrome) or in vitro (eg. cloning or polymerase chain reaction).
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Amplification: An increase in the number of copies of a specific DNA fragment; can either be in vivo or in vitro. See cloning, polymerase chain reaction.
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Gene amplification
Multiple copies of a single gene within a cell.
Gene conversion
An genetic event that produces abnormal segregations by non-reciprocal recombination.
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gene amplification
The selective synthesis of DNA, which results in multiple copies of a single gene, thereby enhancing expression.
gene cloning ...
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Gene Amplification
In Drosophila (fruit flies), the chorion (eggshell) gene is copied many times in certain cells of the oviduct. These cells make large quantities of the protein needed to surround the egg.
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Gene amplification. The presence of multiple genes. Amplification is one mechanism through which proto-oncogenes are activated in malignant cells.
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Signal amplification
A principle of signal transduction is the signal amplification. The binding of one or a few neurotransmitter molecules can enable the entry of millions of ions.
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New DNA amplification technologies developed during the 1990s, such as the polymerase chain reaction (PCR), made obtaining sufficient quantities of DNA much easier.
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(See Dominant gene, Fusion gene, Gene amplification, Gene expression, Gene flow, Gene pool, Gene splicing, Gene translocation, Recessive gene, Regulatory gene.) Gene amplification. The presence of multiple genes.
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Amplification -- any process by which specific DNA sequences are replicated disproportionately greater than their representation in the parent molecules. Aneuploidy -- state of having variant chromosome number (too many or too few). (i.e.
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PCR (Polymerase Chain Reaction) A repetitive procedure that results in a geometric amplification of a specific DNA sequence.
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PCR amplification is possible from complex sequence background e.g. a short sequence from an entire chromosome, and from impure DNA. The technique is widely used in many applications.
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Expression of cathepsin B is regulated at many different levels, from gene amplification, use of alternative promoters, increased transcription and alternative splicing, to increased stability and translatability of transcripts.
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The probable answer: gene amplification. Having multiple copies of genes permits a high level of gene expression; that is, abundant transcription and translation to produce the gene products.
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polymerase chain reaction - powerful DNA synthesis and amplification technique allowing the amplification of a specific sequence from among many others; thoeoretically PCR can be used to detect as little as one specific DNA molecule.
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: One of the more modern methods in cytogenetics, which uses fluorescence-labelled chromosome-specific DNA, probes to detect translocations, inversions, deletions, amplifications and other structural or numerical chromosomal abnormalities.
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Levers amplify or increase the force or velocity of motion. The amount of amplification depends on the length of the lever. There are three types of skeletal system, all interact with muscles using the lever.
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