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Subcloning


In molecular biology, subcloning is a technique used to move a particular gene of interest from a parent vector to a destination vector in order to further study its functionality.
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Easy Subcloning. by Michael Koelle. Subcloning should be easy and fast, and work every time. ... The following protocols minimize the number of manipulations ...
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Essentially all subcloning reactions proceed as follows: You release and purify ... The Subcloning Notebook will lead you through every step in this process. ...
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Our product line includes novel tumor marker proteins, monoclonal antibodies and ... Extensive subcloning is required to selectstable hybrids. ...
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These are in as modular a format as possible my preferred methods for subcloning. ... WHEN NOT TO USE THE FUNDAMENTAL SUBCLONING STRATEGY ...
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Custom Subcloning Service. Subcloning is easier when someone else does it. ... Our subcloning strategies may include any of the following steps: ...
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Chemically competent <I>E. coli</I> cells suitable for subcloning efficiency transformation in a wide variety of applications.<br/><BR><br/><BR><B>Genotype:</B> <I> ...
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The GPS&trade;-1 Genome Priming System provides a simple <I>in vitro</I> method for generating a population of ... walking, random subcloning and nested ...
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One of the most common steps in a molecular cloning experiment is subcloning. ... The overall sequence of a subcloning experiment is in principle quite simple. ...
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tive to CIAP for dephosphorylating DNA for subcloning exper- iments. ... Subcloning Plasmid DNA Constructs. By Bob McLaren, Ph.D. Promega Corporation ...
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Vectors used for genomic, cDNA, and subcloning. General definition. Cloning Vector - intentionally designed artificial DNA construct used ...
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For subcloning optimized human gene sequences (E.coli) ... Convenient subcloning of sequences from QIAgenes Expression Constructs ...
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The subcloning of a DNA fragment from an entry vector into a destination vector ... Schematic overview of the subcloning procedure. ...
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Subcloning PCR Products the TOPO Way. The Scientist 1998, 12 ... I promptly borrowed his TOPO TA Cloning kit and set about subcloning those pesky PCR products. ...
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Subcloning your cDNA of interest ... multiple fragments, unsuitable for subcloning directly. ... digestion strategy or PCR subcloning (see instructions below) ...
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