Navigation Links
His-tag


Contents

Structure

A his-tag consists of approximately six histidine residues near the N- or C-Terminus of a Protein. The total number of histidine residues may vary. It may also be followed by a suitable amino acid sequence that facilitates a removal of the his-tag.

Purpose

Purification

The his-tag can be used in affinity chromatography together with a column that has nickel bound to it. Histidine is a good ligand for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole.

Detection

The his tag can also be used in detection of the protein via anti-his-tag antibodies. This can be useful in localization, ELISA, western blots or other immunoanalytical methods.


'"/>


(Date:3/23/2017)... India , March 23, 2017 The report "Gesture ... Touchless Biometric), Industry, and Geography - Global Forecast to 2022", published by MarketsandMarkets, ... at a CAGR of 29.63% between 2017 and 2022. ... ... ...
(Date:3/22/2017)... 21, 2017 Vigilant Solutions , a ... enforcement agencies, announced today the appointment of retired FBI ... public safety business development. Mr. Sheridan brings ... including a focus on the aviation transportation sector, to ... position, Mr. Sheridan served as the Aviation Liaison Agent ...
(Date:3/13/2017)... Germany , March 13, 2017 Future of security: ... ... DERMALOGs Face Matching enables to match face ... forms the basis to identify individuals. (PRNewsFoto/Dermalog Identification Systems) ... DERMALOG,s "Face Matching" is the fastest software for biometric ...
Breaking Biology News(10 mins):
Other biology definition