Navigation Links
Sequencing


For the sense of "sequencing" used in electronic music, see the music sequencer article.

In genetics and biochemistry, sequencing means to determine the primary structure (or primary sequence) of an unbranched biopolymer. Sequencing results in a symbolic linear depiction known as a sequence which succinctly summarizes much of the atomic-level structure of the sequenced molecule.

In genetics terminology, sequencing is determining the nucleotides of a DNA or RNA strand. Currently, all sequencing is performed using the chain termination method, created by Frederick Sanger. This technique can only be used to identify fairly short sequences at a time (around 300-1000 base pairs on ABI machine), and therefore strategies to have been devised to scale the method up to sequence genes and genome. Two of the mainstream chain termination strategies are chromosome walking and shotgun sequencing.

Other techniques under development, which may offer many benefits over these, include:

Methods for performing protein sequencing include:

Though polysaccharides are also biopolymers, it is not so common to talk of 'sequencing' a polysaccharide, because a symbolic linear depiction cannot capture their tendency to branch and to bond to one another in different ways.

See also

External links



'"/>


(Date:10/15/2014)... book ,Models of Life, is a non-traditional biophysics textbook ... The book is a journey of discovery into biological ... life and biological regulation. It is about how our ... communication between the billions of cells in an organism. ... relationships between principles, which can be found on both ...
(Date:10/15/2014)... will be awarded the 44th Rosenstiel Award for Distinguished ... pioneering research exploring the mechanisms of genomic instability and ... Alt is the second alumnus to win the Rosenstiel ... in 2003. , Alt is the Charles A. Janeway ... Medical School and an investigator at the Howard Hughes ...
(Date:10/14/2014)... house fly genome for the first time, revealing robust ... that thrives in pathogen-rich dung piles and garbage heaps. ... Genome Biology , will increase understanding of house ... adapt to resist insecticides, which could lead to novel ... and transmit more than 100 human and animal diseases, ...
Breaking Biology News(10 mins):New book about life as seen from physics 2Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2House fly genome reveals expanded immune system 2
... Bio-NMR Center at the VIB Department of Molecular and Cellular ... a scientific breakthrough that made it into the leading science ... possible to determine the dynamic structure of proteins. So Flemish ... activity of certain proteins involved in the stress physiology of ...
... 9, 2010) Defeating the flu is challenging because the ... of its genetic code, making it difficult for scientists ... a timely manner. Now, a University of Miami (UM) ... from Stony Brook University have developed a rapid and ...
... most pivotal steps in evolution-the transition from unicellular to ... commonly believed, found a globe-spanning collaboration of scientists led ... and the US Department of Energy,s Joint Genome Institute. ... algae Volvox carteri and its closest unicellular ...
Cached Biology News:Flemish researchers provide the first experimental evidence of dynamic allostery in protein regulation 2Scientists use computer algorithms to develop seasonal flu vaccines 2Origins of multicellularity: All in the family 2Origins of multicellularity: All in the family 3
Other biology definitionOther Tags