Navigation Links
His-tag


Contents

Structure

A his-tag consists of approximately six histidine residues near the N- or C-Terminus of a Protein. The total number of histidine residues may vary. It may also be followed by a suitable amino acid sequence that facilitates a removal of the his-tag.

Purpose

Purification

The his-tag can be used in affinity chromatography together with a column that has nickel bound to it. Histidine is a good ligand for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole.

Detection

The his tag can also be used in detection of the protein via anti-his-tag antibodies. This can be useful in localization, ELISA, western blots or other immunoanalytical methods.


'"/>


(Date:8/28/2014)... an expansion of marine protected areas is needed to ... According to investigators from the Wildlife Conservation Society and ... on saving the largest numbers of species, often at ... difficult-to-replace ecological functions. , Many vital ecological ... that also are food for millions of people. ...
(Date:8/28/2014)... Clinic researcher and his collaborators have developed an online ... process of re-engineering cells for biomedical investigation. CellNet is ... to aid stem cell engineering. Details of CellNet and ... two back-to-back papers in the journal Cell . ... uses for all types of cell-based investigations and can ...
(Date:8/28/2014)... -- Scientists have identified the developmental on-off switch for ... produce more than two-thirds of the world,s naturally derived ... to see whether it is possible to manipulate this ... , The study, appearing August 28 in Cell ... molecule called cyclic-di-GMP and a larger protein called BldD ...
Breaking Biology News(10 mins):Study finds marine protected areas inadequate for protecting fish and ocean ecology 2New tool aids stem cell engineering for medical research 2Small molecule acts as on-off switch for nature's antibiotic factory 2Small molecule acts as on-off switch for nature's antibiotic factory 3
... could perform surgery without ever having to cut through your ... inside the body with a procedure that is as simple ... at the California Institute of Technology (Caltech), all of that ... new method enables researchers to focus light efficiently inside biological ...
... 2012 Polar ( www.polarusa.com ), the leader ... LOOK , the inventor and leader in clipless pedals, ... cycling pedals to measure cycling power output. The Polar LOOK ... output, and send the data directly to a compatible Polar ...
... Charlottesville, VA (June 26, 2012). Researchers from the Department ... and the Department of Integrative Biology and Physiology at ... acid (DHA), an omega-3 fatty acid, and curcumin, a ... injured spinal cord and minimize the clinical and biochemical ...
Cached Biology News:Seeing inside tissue 2Seeing inside tissue 3Seeing inside tissue 4Polar And LOOK Launch The World's First Pedal-Based Power Cycling System 2Polar And LOOK Launch The World's First Pedal-Based Power Cycling System 3Polar And LOOK Launch The World's First Pedal-Based Power Cycling System 4Neuroprotective dietary supplements for chronic spinal cord injury 2Neuroprotective dietary supplements for chronic spinal cord injury 3
Other biology definitionOther Tags