Navigation Links
His-tag


Contents

Structure

A his-tag consists of approximately six histidine residues near the N- or C-Terminus of a Protein. The total number of histidine residues may vary. It may also be followed by a suitable amino acid sequence that facilitates a removal of the his-tag.

Purpose

Purification

The his-tag can be used in affinity chromatography together with a column that has nickel bound to it. Histidine is a good ligand for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole.

Detection

The his tag can also be used in detection of the protein via anti-his-tag antibodies. This can be useful in localization, ELISA, western blots or other immunoanalytical methods.


'"/>


(Date:7/30/2014)... University Press (OUP) is pleased to announce its new ... in 2015, OUP and CINP will partner to publish ... as a fully open-access journal. , Alan Frazer, Editor-in-Chief ... the size and expertise of OUP will further facilitate ... one of the very leading journals within neuropsychopharmacology. The ...
(Date:7/30/2014)... York , July 30, 2014 ... on Global Government Biometric Systems Market 2014-2024 ... The Global Biometric Systems Market 2014-2024" offers ... systems market over the next ten years, alongside ... detailed market size forecasts. To view ...
(Date:7/30/2014)... help Americans make better decisions about what they eat, ... proposed significant changes to the Nutrition Facts label found ... article in Chemical & Engineering News (C&EN), ... explains the suggested updates and the fight that ... C&EN, points out that while the Nutrition Facts label ...
Breaking Biology News(10 mins):OUP to partner with the International College of Neuropsychopharmacology 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 3Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 4Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 5Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 6Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 7Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 8Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 9Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 10
... bird in hand is worth two in the bush may ... the lab of ASU School of Life Sciences Professor Roy ... at the Biodesign Institute. There, associate research scientist ... targets to develop a vaccine against a leading poultry disease, ...
... working gene is inserted into a cell to replace a ... the prevention and treatment of disease. Now a ... a counterintuitive approach also holds promise. The targeted removal of ... would do -- can restore cellular function in cells with ...
... United Kingdom and France have identified four separate ... Creutzfeldt-Jakob disease. The study, published March 14th in ... subgroups could represent distinct prion strains in what ... Prion diseases are transmissible neurodegenerative disorders characterized by ...
Cached Biology News:Solving an avian scourge could also provide benefits to human health 2Solving an avian scourge could also provide benefits to human health 3Work with power grids leads to cell biology discovery 2Work with power grids leads to cell biology discovery 3
Other biology definitionOther Tags